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It is because the bisulfite conversion approach renders the two strands of DNA non-complementary, and in many circumstances primer layout versus just one strand will deliver acceptable primers when the alternative strand will not likely.
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A website large number of PCR primer-style softwares are available on-line. However, only not many of these can be utilized for the look of primers to amplify bisulfite-taken care of DNA templates, vital to determine genomic DNA methylation profiles. Indeed, the volume of scientific studies on bisulfite-addressed templates exponentially will increase as determining DNA methylation gets additional significant within the analysis of cancers. Bisulfite-dealt with DNA is difficult to amplify due to the fact undesired PCR products are frequently amplified due to increased sequence redundancy after the chemical conversion.
Bisulfite conversion of genomic DNA for methylation analysis: protocol simplification with higher Restoration relevant to confined samples and enhanced throughput
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